This gap analysis checklist was developed to be used in the African partner countries that the ABS Capacity Development Initiative is supporting in the national implementation of the Nagoya Protocol and the African Union Guidelines for the Coordinated Implementation of the Nagoya Protocol in Africa (AU Guidelines). This checklist could also be used in other than African countries; In that case, the reference to the AU Guidelines will be obsolete or can be replaced by other applicable regional guidelines. Along with the concept the ABS Initiative can provide already prepared questionnaires to be adapted to national requirements.